how to grow azurescens mushroom indoors

Growing Psilocybe azurescens, also known as “Azurescens mushrooms,” indoors can be challenging because this particular mushroom species is typically found in the wild, particularly along coastal areas in the Pacific Northwest of the United States. However, it is possible to cultivate them indoors if you are experienced with mushroom cultivation and have the right conditions. Keep in mind that the cultivation and use of psilocybin-containing mushrooms are illegal in many places, so be sure to know and follow the laws in your area.

Here’s a general overview of how to grow Psilocybe azurescens indoors:

Materials You’ll Need:

  1. Psilocybe azurescens spore syringe or spore print (if legally obtained)
  2. Substrate (wood chips, straw, or a combination)
  3. Large plastic bags or containers
  4. Perforated bags for fruiting
  5. Sterilization equipment (pressure cooker or autoclave)
  6. Inoculation tools (syringe, scalpel, or flame-sterilized needle)
  7. Growth medium (agar or grain spawn)
  8. Fruiting chamber (humidity-controlled)
  9. Grow lights (optional)
  10. Hygrometer (for monitoring humidity)


  1. Prepare the Substrate: Psilocybe azurescens is a wood-loving mushroom, so it requires a substrate made of wood chips, straw, or a combination. Sterilize the substrate to kill off any competing organisms using a pressure cooker or autoclave.
  2. Inoculate the Substrate: Using a spore syringe or spore print, inoculate the sterilized substrate with Psilocybe azurescens spores or mycelium. You can use aseptic techniques to minimize contamination.
  3. Incubation Stage: Place the inoculated substrate in a large plastic bag or container and seal it. Maintain a temperature of around 70-75°F (21-24°C) for several weeks during the incubation stage. The mycelium will colonize the substrate.
  4. Prepare Grain Spawn or Agar: Some growers prefer to create a grain spawn or agar culture from the mycelium before transferring it to the final substrate. This helps ensure a higher success rate.
  5. Transfer to the Final Substrate: Once the mycelium has colonized the substrate, transfer it to a fruiting chamber. The fruiting substrate should be a mix of wood chips and straw, similar to the initial substrate.
  6. Fruiting Conditions: Psilocybe azurescens requires specific environmental conditions to fruit. These conditions typically involve cooler temperatures (around 50-60°F or 10-16°C), high humidity (90-95%), and indirect light. Monitor and adjust the conditions in the fruiting chamber as needed.
  7. Maintain Humidity: Use a hygrometer to ensure the humidity in the fruiting chamber remains within the required range. Regular misting and air exchange may be necessary to maintain optimal conditions.
  8. Harvesting: Psilocybe azurescens mushrooms will develop over time. Harvest the mushrooms when they are mature but before the caps fully expand. Use sterile tools to remove them from the substrate.
  9. Spore Printing (Optional): If you plan to continue cultivating Psilocybe azurescens, you can make spore prints from mature mushrooms to use for future inoculations.
  10. Dispose of Substrate Properly: When you are finished harvesting, dispose of the spent substrate in an environmentally responsible manner.

Cultivating Psilocybe azurescens mushrooms indoors requires a good understanding of mushroom cultivation, sterile techniques, and specific environmental conditions. It’s essential to research and follow local laws and regulations regarding the cultivation and use of psychoactive mushrooms, as they are illegal in many places.

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